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Lina Polito was terrified when she read the script, especially for nude scenes. Rino Di Silvestro told her, and then had him add on her contract, that she would never shoot completely naked, but in the end, on the screen, she would appear as if she were. She had a little coverage, a triangle on her pubis and stars on her nipples. Silvestro filmed it in such a way that these covers were never seen. And she finally looked naked, but she wasn't.[2]


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FIGURE 5. LTBP4 affected the oncogenicity of melanoma cell in vivo. SK-MEL-1 cells transfected with shRNA-LTBP4, or NC were subcutaneously injected into the nude mice, (A) The nude mice were sacrificed, and the tumors were collected after 32 days; (B) the volume of the tumors were determined; (C) the weight of the tumors were determined; A101D cells transfected with LTBP4 overexpression or NC were subcutaneously injected into the nude mice, (D) The nude mice were sacrificed, and the tumors were collected after 30 days; (E) the volume of the tumors were determined; (F) the weight of the tumors were determined; (G) the expression of Ki67, cleaved caspase-3, Ki67, E-cadherin, YAP, and N-cadherin in the tumors collected from different groups were determined using IHC staining. Data are presented as the mean standard deviation. **p < 0.01 vs NC group. 59ce067264






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